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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NICHD_Hs_Ov1
Organism:Homo sapiens
UniGene Library ID:14438
Tissue Description:
Library Keywords:ovary, normal, bulk, adult, hCGH, full-length enriched, EST, female, FSH, lupron, size fractionated, plasmid vector

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1093

Library Preparation Details

Description:Library is oligo-dT primed and directionally cloned. Granulosa lutein cells aspirated from preovulatory folicles of normal cycling women undergoing ovulation induction for infertility due to male factor and normal doners. The cells were from follicles stimulated with Lupron, FSH and hCG. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 2.23 kb (range 1.0-4.5 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).
R. Site1:SfiI (ggccattatggcc)
R. Site2:SfiI (ggccgcctcggcc)
Lab Host:DH10B (T1 phage-resistant)
Vector Type:plasmid (ampicillin-resistant)
Tissue Supplier:
Library Producer: