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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Lupski_anterior_horn
Organism:Homo sapiens
UniGene Library ID:14298
Tissue Description:Brain, anterior horn, peripheral nervous system
Library Keywords:nerve, normal, non-normalized, bulk, EST, unknown developmental stage, MGC, size fractionated, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:4992
#Sequences Generated to Date:3985

Library Preparation Details

Description:Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 2.1 kb. Library was constructed by Invitrogen and donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine).
R. Site1:EcoRV (destroyed)
R. Site2:NotI
Lab Host:DH10B (T1 phage-resistant)
Vector:pCMV-SPORT6.1
Vector Type:plasmid (ampicillin-resistant)
Tissue Supplier:Dr. James R. Lupski
Library Producer:Invitrogen Corp