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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NIH_MGC_217
Organism:Homo sapiens
UniGene Library ID:14440
Tissue Description:Chondrosarcoma, myxoid chondrosarcoma Grade II, mixture of 3 cell lines, enriched for 0.5-1.0 Kb mRNA
Library Keywords:cartilage, non-normalized, chondrosarcoma, cell line, EST, unknown developmental stage, MGC, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:768
#Sequences Generated to Date:622

Library Preparation Details

Description:Library is oligo-dT primed and directionally cloned Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according tomRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. Average insert size 0.5-1Kb. Adaptors 5'(AATTCGGCACGAGG)3' and 5'd (CCTCGTGCCG)3'. 3' Linker sequence - GCGGCCGCTGAGAGCC T18. Sequencing primers 3'end: T3 promoter primer 5'd (ATTAACCCTCACTAAAGGGA)3'. 5' End: T7 promoter primer 5'd (TAATACGACTCACTATAGGG)3'. Average insert size 0.5-1kb. Library was constructed in the laboratory of M. Bento Soares. Note: this is a NIH_MGC Library.
R. Site1:EcoRI
R. Site2:NotI
Lab Host:DH10B (T1 phage-resistant)
Vector Type:plasmid (ampicillin-resistant)
Tissue Supplier:
Library Producer:M. Bento Soares, University of Iowa