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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NIH_MGC_211
Organism:Homo sapiens
UniGene Library ID:14130
Tissue Description:Prostate cancer
Library Keywords:prostate, cell line, EST, unknown developmental stage, MGC, male, size fractionated, plasmid vector, prostate cancer

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:466

Library Preparation Details

Description:The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pT7T3 Pac vector. The library tag sequence located between the Not I site and the polyA tail is TAGA. Tissue was provided by Tim Ratlift.
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (T1 phage resistant)
Vector:pT7T3 Pac
Vector Type:Plasmid
Tissue Supplier:
Library Producer:M. Bento Soares, University of Iowa