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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Human Lens cDNA (Normalized): fs
Organism:Homo sapiens
UniGene Library ID:13901
Tissue Description:
Library Keywords:normal, bulk, adult, normalized, lens, EST

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:3045

Library Preparation Details

Description:A human lens library (by) was normalized by self-subtraction. One portion of double stranded plasmid DNA representing the library was linearized by NotI. This NotI digested library was used as a template for biotinylated RNA synthesis using SP6 RNA polymerase. Another portion of the double stranded plasmid library was converted to single-stranded circles in vitro using Gene II and Exonuclease III (Life Technologies). Single-stranded DNA (1 mg) was hybridized (C0t 500) with 41 mg of Bio-RNA and vector blocking oligonucleotides. The hybridized Bio-RNA/ss-circles were removed by streptavidin:phenol extraction. EST analysis was performed on the library at the NIH Intramural Sequencing Center(NISC).
R. Site1:
R. Site2:
Lab Host:EMDH10B
Vector:pCMVSPORT6
Vector Type:Plasmid
Tissue Supplier:
Library Producer: