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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:hEx1 (RZPD no. 800)
Organism:Homo sapiens
UniGene Library ID:13865
Tissue Description:
Library Keywords:fetus, normal, bulk, normalized, EST, whole brain

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1430

Library Preparation Details

Description:1st strand cDNA was prepared from mRNA obtained from brain material of two feti, 14.8 and 15.8 weeks old, with a Not I - oligo(dT) primer [5' GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Sal I adaptors, digested with Not I and cloned into the Not I and Sal I sites of the pQE30NST vector. This cDNA expression library represents a subset of clones that were selected for protein expression and has been normalised by oligonucleotide fingerprinting. The library was constructed by Konrad Bussow.
R. Site1:SalI
R. Site2:NotI
Lab Host:
Vector:pQE30NST (AF074376)
Vector Type:Plasmid
Tissue Supplier:
Library Producer: