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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_191
Organism:Homo sapiens
UniGene Library ID:13833
Tissue Description:Blood, peripheral blood mononuclear cells (PBMC) cytoplasmic poly A RNA is pooled sample from 3 and 6 hr after stimulation with PMA and Ionomycin. These reagents are used to activate T- cells that results in lymphokine and cytokine production.
Library Keywords:blood, normal, uncharacterized preparation, peripheral blood mononuclear cell, EST, unknown developmental stage, MGC, PMA, ionomycin, plasmid vector

Clones and Sequences

#Clones Generated to Date:9984
#Sequences Generated to Date:7583

Library Preparation Details

Description:Library is oligo-dT primed and directionally cloned. PBMC - Peripheral Blood Mononuclear Cells. RNA was pooled from 3/6hour stimulation with PMA adn Ionomycin. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.69 kb (range 0.70-5.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.
R. Site1:SfiI (ggccattatggcc)
R. Site2:SfiI (ggccgcctcggcc)
Lab Host:DH10B (T1 phage-resistant)
Vector:pDNR-LIB
Vector Type:plasmid (ampicillin-resistant)
Tissue Supplier:
Library Producer:CLONTECH Laboratories, Inc.