Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
Tissues
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_St3
Organism:Homo sapiens
UniGene Library ID:14437
Tissue Description:Stomach, adenocarcinoma
Library Keywords:stomach, bulk, CGAP, EST, unknown developmental stage, MGC, size fractionated, plasmid vector, directionally cloned, oligo-dT primed, gastric adenocarcinoma

Clones and Sequences

#Clones Generated to Date:1536
#Sequences Generated to Date:1126

Library Preparation Details

Description:Library is oligo-dT primed and directionally cloned. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.30 kb (range 0.60-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).
R. Site1:SfiI (ggccattatggcc)
R. Site2:SfiI (ggccgcctcggcc)
Lab Host:DH10B (T1 phage-resistant)
Vector:pDNR-LIB
Vector Type:plasmid (ampicillin-resistant)
Tissue Supplier:Arnold Schwartz, MD, PhD
Library Producer:CLONTECH