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Cancer Genome Characterization Initiative

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Library Info Page

Library ID

Library Name:subtracted cochlea cDNA library
Organism:Homo sapiens
UniGene Library ID:12768
Tissue Description:
Library Keywords:fetus, normal, cochlea, bulk, subtracted, EST

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:425

Library Preparation Details

Description:Subtraction of cochlea cDNA (tester) was performed with the PCR-Select cDNA Subtraction Kit (Clontech). Driver cDNA consisted of a mixture of cDNAs from brain, kidney, liver, heart, skeletal muscle, placenta, stomach (mucosal layer) and psoas muscle. Tester and driver cDNAs were digested by RsaI; adaptors were ligated to the tester. Two rounds of subtractive hybridization were performed, followed by two suppression PCR reactions. Subtracted PCR products were ligated into a T/A vector (Invitrogen).
R. Site1:
R. Site2:
Lab Host:TOP10F'
Vector Type:
Tissue Supplier:
Library Producer: