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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NIH_MGC_186
Organism:Homo sapiens
UniGene Library ID:13018
Tissue Description:Skin and meninges pool- skin, dura matter, pia matter, choroid plexus.
Library Keywords:brain, spinal cord, skin, vascular, normal, non-normalized, bulk, full-length enriched, EST, unknown developmental stage, mixed tissue, MGC, Clontech non-normalized, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:9984
#Sequences Generated to Date:6795

Library Preparation Details

Description:Library is oligo-dT primed and directionally cloned. cDNA was prepared from a pooled samples of tissues from Skin, meninges, duramatter, pia matter and choroid plexus. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.47 kb (range 0.50-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library
R. Site1:SfiI (ggccattatggcc)
R. Site2:SfiI (ggccgcctcggcc)
Lab Host:DH10B (T1 phage-resistant)
Vector Type:plasmid (chloramphenicol resistant, grown at 30C)
Tissue Supplier:
Library Producer:CLONTECH Laboratories, Inc.