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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_184
Organism:Homo sapiens
UniGene Library ID:12999
Tissue Description:Glandular pool- thyroid, parathyroid, adrenal cortex, pineal gland, submandibular gland.
Library Keywords:adrenal gland, parathyroid, thyroid, normal, non-normalized, cerebral cortex, bulk, pineal gland, full-length enriched, EST, unknown developmental stage, MGC, Clontech non-normalized, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:9600
#Sequences Generated to Date:7495

Library Preparation Details

Description:Library is oligo-dT primed and directionally cloned. cDNA was prepared from a glandular pool of tissues from thyoid, parathyroid, adrenal, cortex and pineal gland. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.38 kb (range 0.60-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.
R. Site1:SfiI (ggccattatggcc)
R. Site2:SfiI (ggccgcctcggcc)
Lab Host:DH10B (T1 phage-resistant)
Vector:pDNR-LIB
Vector Type:plasmid (chloramphenicol resistant, grown at 30C)
Tissue Supplier:
Library Producer:CLONTECH Laboratories, Inc.