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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_183
Organism:Homo sapiens
UniGene Library ID:14414
Tissue Description:Muscle pool- 2 tissues- cardiac and skeletal muscle.
Library Keywords:muscle, normal, non-normalized, bulk, EST, LTI non-normalized, unknown developmental stage, MGC, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:1536
#Sequences Generated to Date:1295

Library Preparation Details

Description:Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7. Library was constructed by Invitrogen.
R. Site1:EcoRV (destroyed)
R. Site2:NotI
Lab Host:DH10B-TonA (T1 and T5 phage resistant)
Vector:pCMV-SPORT6.1
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer:Invitrogen Corp