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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:L2HLK1
Organism:Homo sapiens
UniGene Library ID:12546
Tissue Description:
Library Keywords:liver, cell line, EST, unknown developmental stage, male, plasmid vector, liver tumor

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:128

Library Preparation Details

Description:The poly (A)+ RNA was dephosphorylated with bacterial alkaline phosphatase (BAP) and then decapped with tabacco acid pyrophosphatase (TAP). The decapped intact mRNA was ligated with DNA-RNA linker including SfiI site by treatment of T4 RNA ligase and the first strand cDNA was synthesized with Superscript II using SfiI oligo-dT primer. After first strand synthesis, RNA was degraded by NaOH treatment and cDNA was amplified by PCR reaction. The PCR products were digested with SfiI and cloned into DraIII-digested pME18S-FL3 vector. The obtained cDNA vectors were used for transformation of competent cells E. coli Top10F' by electroporation method. The cDNA libraries constructed by this method are full-length enriched cDNA library.
R. Site1:XhoI
R. Site2:XhoI
Lab Host:Top10F'
Vector:pME18S-FL3
Vector Type:Plasmid
Tissue Supplier:
Library Producer: