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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Schneider fetal brain 00004
Organism:Homo sapiens
UniGene Library ID:1918
Tissue Description:
Library Keywords:fetus, normal, cerebrum, bulk, full-length enriched, EST, anterior, male, size fractionated, plasmid vector

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:7899

Library Preparation Details

Description:Double-stranded cDNA was prepared from human fetal brain tissue. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-GAGAGAGAGAAGAGCTCAAGGATCCTTAATTAAATTAATCCCCCCCCCCCCC-3' and 3' adaptor sequence: 5'-GAGAGAGAGACTCGAGTTTTTTTTTTTTTTTTTT-3'. The library was size-selected for >0.5 kb inserts and has an average insert size estimated at 1.2 kb. This library was constructed using the CAP-trapper method for full-length enrichment and has not undergone amplification. Library was constructed by Dr. Claudio Schneider (LNCIB-Area Science Park, Trieste, Italy).
R. Site1:SstI
R. Site2:XhoI
Lab Host:DH10B
Vector:pBluescript SK (Stratagene)
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: