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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Ov18
Organism:Homo sapiens
UniGene Library ID:2299
Tissue Description:Ovary, fibrothecoma
Library Keywords:ovary, fibrothecoma, bulk, normalized, CGAP, EST, Soares normalized, unknown developmental stage, female, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:13824
#Sequences Generated to Date:6963

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCGACATTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (phage-resistant)
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Christopher A. Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Soares Laboratory