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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Human Retinal pigment epithelium/choroid cDNA (Un-normalized, unamplified): cs
Organism:Homo sapiens
UniGene Library ID:12093
Tissue Description:
Library Keywords:eye, normal, non-normalized, adult, pigmented epithelium, EST

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:9662

Library Preparation Details

Description:Two different donor eyes (75-80 years old) yielded approximately 600 mg of dissected RPE/choroid tissue. This in turn yielded 340 ug of total RNA and 7 ug of mRNA. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies (Rockville, MD; now part of Invitrogen Corp), essentially following the protocols of the SuperScript Plasmid System (Invitrogen Corp. ). The library code designation was cs. For this library, cDNA inserts were cloned into the NotI/MluI sites of the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC).
R. Site1:
R. Site2:
Lab Host:EMDH10B
Vector:pCMVSPORT6
Vector Type:Plasmid
Tissue Supplier:
Library Producer: