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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NCI_CGAP_FT1
Organism:Homo sapiens
UniGene Library ID:12072
Tissue Description:Lung, alveolar macrophage
Library Keywords:lung, uncharacterized histology, bulk, adult, normalized, macrophage, CGAP, EST, Soares normalized, MGC, stress, plasmid vector, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:11136
#Sequences Generated to Date:9502

Library Preparation Details

Description:NCI_CGAP_FT1 is a normalized cDNA library constructed from a pool of 81 RNA samples from Alveolar Macrophages challenged with different treatments. The mRNA samples were a mixture of these conditions (times refer to incubations following isolation by bronchoalveolar lavage) (some normal donor macrophages were cultured in some of the conditions, other donor macrophages in different conditions). The mRNA samples were pooled for library construction. Control 0 hours; control 3 hours; control 24 hours; LPS 100 ng/ml, 3 hours; LPS 100 ng/ml, 24 hours; PMA 10 ng/ml, 3 hours; PMA 10 ng/ml, 24 hours; Klebsiella moi 10, 3 hours; Klebsiella moi 10, 24 hours; Staph aureus moi 10, 3 hours; Staph aureus moi 10, 24 hours; Adenoviral vector (Ad5 CMV eGFP), moi 500, 3 hours; Adenoviral vector (Ad5 CMV eGFP), moi 500, 24 hours; wt adenovirus moi 500, 3 hours; wt adenovirus moi 500, 24 hours; Ad vector + LPS 3 hours; Ad vector + LPS 24 hours; wt adenovirus + LPS 3 hours; wt adenovirus + LPS 24 hours. The library was normalized according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GGCCATGCCG. The tissue was provided by Dr. Gary W. Hunninghake of the University of Iowa.
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (Life Technologies)
Vector:pT7T3-Pac (Pharmacia) with a modified polylinker
Vector Type:Plasmid
Tissue Supplier:Dr. Gary W. Hunninghake of the University of Iowa
Library Producer:Soares Laboratory