Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
Tissues
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Hembase; Erythroid Precursor Cells (LCB:cl library)
Organism:Homo sapiens
UniGene Library ID:11923
Tissue Description:
Library Keywords:blood, normal, cell line, peripheral blood mononuclear cell, EST, unknown developmental stage, size fractionated, plasmid vector

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:9842

Library Preparation Details

Description:A complementary DNA (cDNA) library from human erythroid precursor cells was constructed using SMART PCR (polymerase chain reaction) cDNA Library Construction Kit (Clontech, Palo Alto, CA) according to the manufacturer's directions, but with slight modifications. Briefly, reverse transcription was performed in the presence of 1 umol/L peptide nucleic acid (PNA) oligos (N-terminal)-biotin-GTC-CAC-CCG-AAG-CTT-G-(C-terminal) and (N-terminal)-biotin-C(T/C)T-GAA-GTT-CTC-AGG-A-(C-terminal). Synthesized cDNA was digested with SfiI and size-selected on a 1% agarose gel (>800bp). Large-scale sequencing of the library was performed by the NIH Intramural Sequencing Center (NISC; Http://www.nisc.nih.gov/).
R. Site1:SfiI
R. Site2:SfiI
Lab Host:DH5alpha
Vector:pTriplEx2
Vector Type:plasmid
Tissue Supplier:
Library Producer: