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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_FL1
Organism:Homo sapiens
UniGene Library ID:11917
Tissue Description:Bone, human Chondrosarcoma Grade 3 cell line mix
Library Keywords:bone, chondrosarcoma, cell line, adult, normalized, CGAP, EST, Soares normalized, grade III, MGC, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:6144
#Sequences Generated to Date:5221

Library Preparation Details

Description:NCI_CGAP_FL1 is a normalized cDNA library derived from a pool of mRNA obtained from 4 cell lines from grade III chondrosarcoma tissues. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GAGGTCGGTG. The cell lines were provided by Dr. James Martin from the University of Iowa.
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (Life Technologies)
Vector:pT7T3-Pac (Pharmacia) with a modified polylinker
Vector Type:Plasmid
Tissue Supplier:James Martin
Library Producer:Soares Laboratory