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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_148
Organism:Homo sapiens
UniGene Library ID:13001
Tissue Description:Placenta, pre-eclamptic
Library Keywords:normal, placenta, bulk, pregnant, adult, normalized, EST, MGC, female, size fractionated, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:11520
#Sequences Generated to Date:8633

Library Preparation Details

Description:Library is oligo-dT primed and directionally cloned using primer 5'-TTTTTTTTTTTTTTTTVN-3", size-selected for average insert size 2.3 kb and normalized to ROT 5. This is a primary library enriched for full-lenght clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: this is a NIH_MGC Library.
R. Site1:alI-XhoI
R. Site2:BamH
Lab Host:DH10B TonA
Vector:pBluescriptR
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer:Michael Brownstein / Ted Usdin Laboratory