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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NIH_MGC_147
Organism:Homo sapiens
UniGene Library ID:13000
Tissue Description:Placenta, normal
Library Keywords:normal, placenta, bulk, pregnant, adult, normalized, EST, MGC, female, size fractionated, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:11904
#Sequences Generated to Date:8121

Library Preparation Details

Description:Oligo-dT primed using primer 5'-TTTTTTTTTTTTTTTTVN-3', size-selected for average insert size 2.3 kb and normalized to ROT 5. This is a primary library enriched for full-length clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: This is a NIH_MGC library.
R. Site1:alI-XhoI
R. Site2:BamH
Lab Host:DH10B TonA
Vector:pBluescriptR
Vector Type:phagemid (ampicillin resistant
Tissue Supplier:
Library Producer:Michael Brownstein / Ted Usdin Laboratory