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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:HEV PCR-select
Organism:Homo sapiens
UniGene Library ID:10376
Tissue Description:
Library Keywords:normal, endothelium, normalized, subtracted, antibody purified, EST, tonsil, unknown developmental stage, plasmid vector

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1049

Library Preparation Details

Description:High endothelial cells (HEC) were isolated from human tonsils using magnetic beads derivatized with MECA-79 antibody specific to the peripheral lymph node addressin. cDNA was prepared from these cells using Cap-Finder methodology, then cDNA prepared from tonsillar lymphocytes and from human umbilical vein endothelial cells were subtracted from the HEC cDNA, followed by normalization through selective PCR amplification.
R. Site1:EcoRI
R. Site2:EcoRI
Lab Host:
Vector:pCRII
Vector Type:Plasmid
Tissue Supplier:
Library Producer: