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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NCI_CGAP_Pl5
Organism:Homo sapiens
UniGene Library ID:10423
Tissue Description:Placenta, human full term placenta derived from NCI_CGAP_Pl4
Library Keywords:normal, placenta, bulk, pregnant, adult, subtracted, CGAP, EST, Soares subtracted, female, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:647

Library Preparation Details

Description:NCI_CGAP_Pl5 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are GA, AGGAA. For additional information, contact: Bento Soares, bento-soares@uiowa.edu
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (Life Technologies)
Vector:pT7T3-Pac (Pharmacia) with a modified polylinker
Vector Type:Plasmid
Tissue Supplier:Dr. Stephen Brown
Library Producer:Soares Laboratory