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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NCI_CGAP_Fs1
Organism:Homo sapiens
UniGene Library ID:10418
Tissue Description:Fibrosarcoma
Library Keywords:connective tissue, fibroblast, non-normalized, cell line, adult, CGAP, EST, Soares non-normalized, fibrosarcoma, MGC, male

Clones and Sequences

#Clones Generated to Date:5760
#Sequences Generated to Date:4100

Library Preparation Details

Description:NCI_CGAP_Fs1 is a cDNA library containing the following tissue(s): Fibrosarcoma Cell line HT-1088 (ATCC number CCL-121). The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GTTCTACGAG.
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (Life Technologies)
Vector:pT7T3-Pac (Pharmacia) with a modified polylinker
Vector Type:Plasmid
Tissue Supplier:Dr. Mary Hendrix
Library Producer:Soares Laboratory