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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:S5SNU484
Organism:Homo sapiens
UniGene Library ID:10301
Tissue Description:
Library Keywords:stomach, cell line, EST, unknown developmental stage, plasmid vector, stomach cancer

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:2552

Library Preparation Details

Description:The poly (A)+ RNA was decapped with tabacco acid pyrophosphatase (TAP) and ligated with DNA-RNA linker including EcoRI site by treatment of T4 RNA ligase. The first strand cDNA was synthesized from oligo dT-selected mRNA by priming with dT-tailed vector. The dT-tailed vector was adjusted to have about 60nt. The cDNA vector was circularized with E. coli DNA ligase after digestion of EcoRI which site is also included in vector. An RNA strand converted to a DNA strand by Okayama-Berg method. The obtained cDNA vectors were used for transformation of competent cells E. coli Top10F' by electroporation method.
R. Site1:EcoRI
R. Site2:NotI
Lab Host:Top10F'
Vector Type:Plasmid
Tissue Supplier:
Library Producer: