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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:UI-E-CI0
Organism:Homo sapiens
UniGene Library ID:10286
Tissue Description:
Library Keywords:eye, normal, bulk, adult, pigmented epithelium, EST, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1269

Library Preparation Details

Description:UI-E-CI0 is a cDNA library containing the following tissue(s): RPE and Choroid. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is ACCTA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (Life Technologies) (T1 phage resistant)
Vector:pT7T3-Pac (Pharmacia) with a modified polylinker
Vector Type:Plasmid
Tissue Supplier:
Library Producer: