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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:UI-E-DX1
Organism:Homo sapiens
UniGene Library ID:10278
Tissue Description:
Library Keywords:fetus, eye, normal, bulk, EST, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:3467

Library Preparation Details

Description:UI-E-DX1 is a normalized cDNA library containing the following tissue(s): fetal eyes. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGAATCAAGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI).
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (Life Technologies) (T1 phage resistant)
Vector:pT7T3-Pac (Pharmacia) with a modified polylinker
Vector Type:Plasmid
Tissue Supplier:
Library Producer: