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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_DF1
Organism:Homo sapiens
UniGene Library ID:10409
Tissue Description:Bone, subchondral
Library Keywords:bone, normal, bulk, adult, normalized, CGAP, EST, Soares normalized, MGC, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:2304
#Sequences Generated to Date:1923

Library Preparation Details

Description:NCI_CGAP_DF1 is a normalized cDNA library containing the following tissue(s): Subchondral Bone. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is GTTAAGCGTC.
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (Life Technologies)
Vector:pT7T3-Pac (Pharmacia) with a modified polylinker
Vector Type:Plasmid
Tissue Supplier:Dr. Jose Mercuende
Library Producer:Soares Laboratory