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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_125
Organism:Homo sapiens
UniGene Library ID:10196
Tissue Description:Ovary, pooled from 3 adults
Library Keywords:ovary, normal, bulk, adult, normalized, full-length enriched, EST, LTI normalized, MGC, female, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:9600
#Sequences Generated to Date:4722

Library Preparation Details

Description:RNA source pool of three ovaries, from females ranging in age from 38 to 49 yo. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 2.1 kb, insert size range 1-3.5 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 036.
R. Site1:EcoRV (destroyed)
R. Site2:NotI
Lab Host:DH10B
Vector:pCMV-SPORT6
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer:Life Technologies, Inc.