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Library Info Page


Library ID

Library Name:NIH_MGC_124
Organism:Homo sapiens
UniGene Library ID:10197
Tissue Description:Brain, hippocampus
Library Keywords:normal, hippocampus, bulk, adult, normalized, full-length enriched, EST, LTI normalized, MGC, male, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:9600
#Sequences Generated to Date:4522

Library Preparation Details

Description:RNA source male hippocampus, age 27. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 0.9-4 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 012.
R. Site1:EcoRV (destroyed)
R. Site2:NotI
Lab Host:DH10B
Vector:pCMV-SPORT6
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer:Life Technologies, Inc.