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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Melton Normalized Human Islet 4 N4-HIS 1
Organism:Homo sapiens
UniGene Library ID:9821
Tissue Description:
Library Keywords:pancreatic islet, normal, bulk, adult, normalized, EST, size fractionated, plasmid vector, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:18702

Library Preparation Details

Description:Starting library constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA made by oligo-dT priming. Size-selected by column fractionation; average insert size 1.08 kb. Library was amplified once on solid support and plasmid DNA from library was prepared. The library DNA was normalized by method #4 from Bonaldo, Lennon, and Soares 1996 Genome Research 6:791-806; 0.5 microgram single-stranded library plasmid DNA was mixed with 5 micrograms PCR product representing library inserts and hybridized to an Ecot of 20. Single-stranded (unhybridized) plasmids were isolated by hydroxyapatite chromatography and used to make this library.
R. Site1:Not 1
R. Site2:Sal 1
Lab Host:DH10B
Vector:pSPORTl
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: