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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Human insulinoma
Organism:Homo sapiens
UniGene Library ID:9716
Tissue Description:
Library Keywords:pancreatic islet, pancreas, bulk, EST, unknown developmental stage, phagemid, insulinoma

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:33390

Library Preparation Details

Description:Constructed with lambda ZAPII system (Stratagene) by Dr. J. Ferrer, in vivo mass-excised to pBluescript SK- by Dr. H. Inoue following the Washington University protocol (http://genome.wustl.edu/est/lambda_protocol.shtml). Please contact Hiroshi Inoue, MD/PhD for further information on this library (Metabolism Division, Permutt Laboratory, Washington University School of Medicine, Box 8127, 660 S Euclid Ave, St. Louis, MO 63110). Note: this is a Washington University Pancreas EST project library.
R. Site1:XhoI
R. Site2:EcoRI
Lab Host:DH10B (phage-resistant)
Vector:pBluescript SK-
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: