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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_121
Organism:Homo sapiens
UniGene Library ID:9727
Tissue Description:Brain, fetal, whole pooled
Library Keywords:fetus, brain, normal, bulk, normalized, full-length enriched, EST, LTI normalized, MGC, mixed pool by gender, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:13440
#Sequences Generated to Date:9778

Library Preparation Details

Description:RNA source anonymous pool of 3 fetal brains, female age 20 weeks, female age 24 weeks, and male age 26 weeks. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7 kb, insert size range 0.7-3.5 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 017. Note: this is a NIH_MGC Library.
R. Site1:NotI
R. Site2:EcoRV (destroyed)
Lab Host:DH10B
Vector:pCMV-SPORT6
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer:Life Technologies, Inc.