Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
Tissues
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NIH_MGC_119
Organism:Homo sapiens
UniGene Library ID:9725
Tissue Description:Brain, adult medulla
Library Keywords:normal, bulk, adult, normalized, medulla oblongata, full-length enriched, EST, LTI normalized, MGC, male, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:13056
#Sequences Generated to Date:10788

Library Preparation Details

Description:RNA source normal medulla from anonymous male age 27. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.3 kb, insert size range 0.9-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 013. Note: this is a NIH_MGC Library.
R. Site1:NotI
R. Site2:EcoRV (destroyed)
Lab Host:DH10B
Vector:pCMV-SPORT6
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer:Life Technologies, Inc.