Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
Tissues
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NIH_MGC_116
Organism:Homo sapiens
UniGene Library ID:9723
Tissue Description:Colon, Kidney, Stomach, adult, whole pooled
Library Keywords:kidney, stomach, normal, colon, bulk, adult, normalized, full-length enriched, EST, LTI normalized, MGC, mixed pool by gender, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:13440
#Sequences Generated to Date:10271

Library Preparation Details

Description:RNA source anonymous pool of 3 colons, age 26 yo male, 49 yo female, 71 yo male colon; 46 yo male kidney, and pool of 2 stomachs, 62 yo male and 70 yo female. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 023. Note: this is a NIH_MGC Library.
R. Site1:NotI
R. Site2:EcoRV (destroyed)
Lab Host:DH10B
Vector:pCMV-SPORT6
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer:Life Technologies, Inc.