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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NIH_MGC_114
Organism:Homo sapiens
UniGene Library ID:9721
Tissue Description:Brain, adult, 6 pooled whole brains
Library Keywords:brain, normal, bulk, adult, normalized, full-length enriched, EST, LTI normalized, MGC, male, size fractionated, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:13440
#Sequences Generated to Date:9521

Library Preparation Details

Description:RNA source anonymous pool of 6 male brains, age range 23-27 yo. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.5 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 019. Note: this is a NIH_MGC Library.
R. Site1:NotI
R. Site2:EcoRV (destroyed)
Lab Host:DH10B
Vector:pCMV-SPORT6
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer:Life Technologies, Inc.