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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Hembase; Erythroid Progenitor Cells (LCB:ax library)
Organism:Homo sapiens
UniGene Library ID:8975
Tissue Description:
Library Keywords:blood, normal, cell line, flow-sorted, erythropoietin, peripheral blood mononuclear cell, EST, unknown developmental stage

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:5182

Library Preparation Details

Description:65,000 proliferating erythroid cells from the buffy coat of a blood donation were obtained by flow cytometric separation after a 5-day culture period in the presence of erythropoietin. Total RNA was purified from the sorted cell population using TRIzol reagent. RNA (0.3 ug) was converted into double stranded cDNA using Clontech's CapFinder cDNA Library Construction Kit (Clontech) according to the manufacturer's protocol and cloned into EcoRI digested Lambda Zap II vector (Stratagene). The phage library was amplified once prior to in vivo excision in SOLR cells. Individual colonies were grown, and the cDNA inserts were sequenced in high throughput (NIH intramural sequencing center http://www.nisc.nih.gov/).
R. Site1:EcoRI
R. Site2:EcoRI
Lab Host:SOLR
Vector:Lambda ZAP II
Vector Type:plasmid
Tissue Supplier:
Library Producer: