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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Sub9
Organism:Homo sapiens
UniGene Library ID:10426
Tissue Description:Pooled, mixture of 9 tissues. Subtraction drivers: NCI_CGAP_Kid3, NCI_CGAP_Kid5, NCI_CGAP_Lu5, NCI_CGAP_GC4, NCI_CGAP_Pr22, NCI_CGAP_Co10, NCI_CGAP_Sub1-7
Library Keywords:uncharacterized tissue, uncharacterized histology, bulk, subtracted, CGAP, EST, Soares subtracted, mixed developmental stages

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:3915

Library Preparation Details

Description:tissues: Cholonic mucosa with Crohns disease, Cholonic mucosa with ulcerative colitis, Fetal thymus, Cervix, Cervical adenosquamous carcinoma, Ligament cells, Prostate carcinoma, Bladder carcinoma, Brain oligodenroga ; NCI_CGAP_Sub9 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are CGTC, AACG, GGGCC, GGAAG, TAGC, TAAGC, ATGG, AGACA, ATCAC. For additional information, contact: Bento Soares, bento-soares@uiowa.edu
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (Life Technologies)
Vector:pT7T3-Pac (Pharmacia) with a modified polylinker
Vector Type:Plasmid
Tissue Supplier:Dr. Brian Dieckgraefe, Dr. David Lewis, Dr. Daniela Gerhard, Jeff Stevens, Tim Ratlift, Bob Strausberg
Library Producer:Soares Laboratory