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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Lupski_sympathetic_trunk
Organism:Homo sapiens
UniGene Library ID:7227
Tissue Description:Peripheral Nervous System, sympathetic trunk
Library Keywords:nerve, normal, non-normalized, bulk, juvenile, EST, LTI non-normalized, MGC, male, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:16896
#Sequences Generated to Date:9506

Library Preparation Details

Description:cDNA made by oligo-dT priming. Directionally cloned using the following adaptors: 5'-TCGACCCACGCGTCCG-3' and 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size selected > 1 kb for average insert length 1.9 kb. This is a primary library, non-amplified. Library constructed by Life Technologies and donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine); available through Life Technologies.
R. Site1:NotI
R. Site2:SalI
Lab Host:DH10B
Vector:pCMV-SPORT6 (Life Technologies)
Vector Type:plasmid (ampicillin resistant, 200 ug/ml)
Tissue Supplier:Dr. James R. Lupski
Library Producer:Life Technologies, Inc.