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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NIH_MGC_97
Organism:Homo sapiens
UniGene Library ID:8571
Tissue Description:Testis
Library Keywords:testis, normal, bulk, normalized, full-length enriched, EST, unknown developmental stage, MGC, male, size fractionated, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:33097
#Sequences Generated to Date:21470

Library Preparation Details

Description:Oligo-dT primed using primer 5'-TTTTTTTTTTTTTTTTVN-3', size-selected for average insert size 2.2 kb and normalized to ROT 5. This is a primary library enriched for full-length clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: this is a NIH_MGC Library.
R. Site1:BamHI
R. Site2:SalI-XhoI (gtcgag)
Lab Host:DH10B
Vector:pBluescriptR (modified pBluescript KS+)
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer:Michael J. Brownstein (NHGRI) & Shiraki Toshiyuki and Piero Carninci (RIKEN)