Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NCI_CGAP_Pr2
Organism:Homo sapiens
UniGene Library ID:574
Tissue Description:Prostate prostatic intraepithelial neoplasia
Library Keywords:prostate, low grade, non-normalized, microdissected, adult, CGAP, EST, Krizman protocol 1, male, oligo-dT primed, prostatic intraepithelial neoplasia

Clones and Sequences

#Clones Generated to Date:8448
#Sequences Generated to Date:5931

Other Libraries from Same Tissue Sample


Library Preparation Details

Description:1st strand cDNA was primed with oligo(dT)17 on 50 ng of DNAse-treated, total cellular RNA obtained from 5,000-10,000 microdissected preneoplastic cells histologically-determined to be prostatic intraepithelial neoplasia 2 (PIN2) cells. Double-stranded cDNA was ligated to EcoRI adaptors, 5 cycles of PCR applied to the cDNA with an adaptor-specific primer, and the resulting PCR product subcloned into pAMP10 by the UDG-cloning method (Life Technologies). Average insert size is 600 bp. NOTE: Not directionally cloned. This library was constructed by David Krizman.
R. Site1:Not1
R. Site2:EcoRI
Lab Host:DH10B
Vector Type:Phagemid (ampicillin resistant)
Tissue Supplier:W. Marston Linehan, M.D., Rodrigo Chuaqui, M.D., Michael Emmert-Buck, M.D., Ph.D.
Library Producer:Krizman Laboratory