Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
Tissues
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Brn65
Organism:Homo sapiens
UniGene Library ID:8619
Tissue Description:Brain, glioblastoma without EGFR amplification
Library Keywords:brain, glioblastoma multiforme, non-normalized, bulk, CGAP, EST, LTI non-normalized, unknown developmental stage, size fractionated, directionally cloned, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:1920
#Sequences Generated to Date:1156

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.77 kb. Constructed by Life Technologies.
R. Site1:NotI
R. Site2:SalI
Lab Host:DH10B (T1 phage-resistant)
Vector:pCMV-SPORT6
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:David N. Louis, M.D.
Library Producer:Life Technologies, Inc.