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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_77
Organism:Homo sapiens
UniGene Library ID:6834
Tissue Description:Lung
Library Keywords:lung, normal, non-normalized, bulk, full-length enriched, EST, unknown developmental stage, MGC, Clontech non-normalized, size fractionated, plasmid vector

Clones and Sequences

#Clones Generated to Date:20736
#Sequences Generated to Date:18981

Library Preparation Details

Description:5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.9 kb (range 0.5-4.0 kb). 12/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.
R. Site1:SfiI (ggccgcctcggcc)
R. Site2:SfiI (ggccattatggcc)
Lab Host:DH10B (T1 phage-resistant)
Vector:pDNR-LIB (Clontech)
Vector Type:plasmid (chloramphenicol resistant, grown at 30C)
Tissue Supplier:
Library Producer:CLONTECH Laboratories, Inc.