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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NIH_MGC_48
Organism:Homo sapiens
UniGene Library ID:6987
Tissue Description:Primary B-Cells from Tonsils
Library Keywords:B-cell, normal, non-normalized, cell line, EST, tonsil, unknown developmental stage, MGC, Rubin non-normalized, size fractionated, plasmid vector

Clones and Sequences

#Clones Generated to Date:20352
#Sequences Generated to Date:18479

Library Preparation Details

Description:cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.
R. Site1:XhoI
R. Site2:EcoRI
Lab Host:DH10B (phage-resistant)
Vector:pOTB7
Vector Type:plasmid (chloramphenicol resistant, grown at 30C)
Tissue Supplier:
Library Producer:Rubin Laboratory