Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
Tissues
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NIH_MGC_83
Organism:Homo sapiens
UniGene Library ID:6763
Tissue Description:Prostate
Library Keywords:prostate, normal, non-normalized, bulk, full-length enriched, EST, unknown developmental stage, MGC, Clontech non-normalized, male, size fractionated, plasmid vector

Clones and Sequences

#Clones Generated to Date:15744
#Sequences Generated to Date:10900

Library Preparation Details

Description:5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.4 kb (range 0.5-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).
R. Site1:SfiI (ggccgcctcggcc)
R. Site2:SfiI (ggccattatggcc)
Lab Host:DH10B (T1 phage-resistant)
Vector:pDNR-LIB (Clontech)
Vector Type:plasmid (chloramphenicol resistant, grown at 30C)
Tissue Supplier:
Library Producer:CLONTECH Laboratories, Inc.