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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Hembase; Erythroid Progenitor Cells (LCB:ad library)
Organism:Homo sapiens
UniGene Library ID:1731
Tissue Description:
Library Keywords:blood, normal, cell line, subtracted, peripheral blood mononuclear cell, EST, unknown developmental stage

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:721

Library Preparation Details

Description:Human peripheral blood mononuclear cells were cultured in the presence or absence of erythropoietin. CD71++++ cells arising only in erythropoietin-supplemented medium were isolated by fluorescence activated cell sorting. Those cells demonstrated an average of six additional doublings in suspension culture and erythroid colony formation in methylcellulose. Suppression subtractive hybridization was used to construct the ad library (tester=sorted CD71++++ cells, driver=unsorted cells cultured without erythropoietin).
R. Site1:EcoRI
R. Site2:EcoRI
Lab Host:DH5alpha
Vector:pCRII
Vector Type:plasmid
Tissue Supplier:
Library Producer: