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Library Info Page


Library ID

Library Name:Soares_Dieckgraefe_colon_NHCD
Organism:Homo sapiens
UniGene Library ID:1728
Tissue Description:Colon, colonic mucosa from 3 patients with Crohn's disease
Library Keywords:epithelium, Crohn's disease, colon, bulk, normalized, CGAP, EST, Soares normalized, unknown developmental stage, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:1920
#Sequences Generated to Date:3164

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGTCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Tissue samples provided by Dr. Brian Dieckgraefe (Washington University, dieck@im.wustl.edu); colonic mucosa represents a range of disease involvement from moderate to severe Crohn's disease; samples include both perforating (fistulas) and non-perforating samples. Library constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (phage-resistant)
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Dr. Brian Dieckgraefe
Library Producer:Soares Laboratory