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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NCI_CGAP_Sub8
Organism:Homo sapiens
UniGene Library ID:7239
Tissue Description:Pooled, a mixture of 21 normalized or subtracted NCI_CGAP libraries. NCI_CGAP_Co4, Pr22, Pr28, Co10, Co16, Kid5, Kid12, Kid3, Kid11, Lym2, Br2, Co8, CLL1, Lei2, Brn23, Lu5, Lu24, Lu19, GC4, GC6, Brn25
Library Keywords:uncharacterized tissue, uncharacterized histology, bulk, subtracted, CGAP, EST, Soares subtracted, unknown developmental stage, MGC

Clones and Sequences

#Clones Generated to Date:1152
#Sequences Generated to Date:4295

Other Libraries from Same Tissue Sample


Library Preparation Details

Description:NCI_CGAP_Sub8 is a subtracted library derived from NCI_CGAP_Sub5. The NCI_CGAP_Sub8 library had 2.5 million recombinants. A single-stranded DNA preparation of NCI_CGAP_Sub5 was used as a tracer in a subtractive hybridization with a driver comprising: a pool of clones from NCI_CGAP_Sub5 (IMAGE clone Ids 2732833-2737415, 3068040-3069191; 25% of the driver population), a pool of clones from NCI_CGAP_Sub4 (IMAGE clone Ids 2723592-2729326; 25% of the driver population), NCI_CGAP_Sub6 (pool AIF-AJU, IMAGE Ids 2728969-2733190; 25% of the driver population), and NCI_CGAP_Sub7 (IMAGE Ids 3069192-3072238, 3081864-3084550; 25% of the driver population). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (Life Technologies)
Vector Type:Phagemid
Tissue Supplier:
Library Producer:Soares Laboratory