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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Human Epidermal Keratinocyte Subtraction Library- Upregulated Transcripts
Organism:Homo sapiens
UniGene Library ID:5122
Tissue Description:
Library Keywords:skin, cell line, adult, subtracted, sulfur mustard, EST, female, plasmid vector

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:596

Library Preparation Details

Description:Library preparation: Sequence isolated using PCR-select (Clontech) subtraction library construction (Diatchenko et al., (1996) Proc. Natl. Acad. Sci. USA 93: 6025-6030) after sulfur mustard exposure of primary human adult epidermal keratinocytes. Subtraction library cloned for random sequence selection into pT-Adv vector.
R. Site1:
R. Site2:
Lab Host:E. coli (ampicillin-resistant)
Vector Type:Plasmid
Tissue Supplier:
Library Producer: