Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NIH_MGC_60
Organism:Homo sapiens
UniGene Library ID:6831
Tissue Description:Prostate, adenocarcinoma.
Library Keywords:prostate, non-normalized, cell line, adult, full-length enriched, EST, MGC, Clontech non-normalized, male, size fractionated, plasmid vector, prostatic adenocarcinoma

Clones and Sequences

#Clones Generated to Date:14208
#Sequences Generated to Date:10954

Library Preparation Details

Description:Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.5 kb (range 0.9-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.
R. Site1:SfiI (ggccgcctcggcc)
R. Site2:SfiI (ggccattatggcc)
Lab Host:DH10B (T1 phage-resistant)
Vector:pDNR-LIB (Clontech)
Vector Type:plasmid (chloramphenicol resistant, grown at 30C)
Tissue Supplier:
Library Producer:CLONTECH Laboratories, Inc.